Experimental Pathology Research Laboratory

Covid update

All researchers visiting the Core must wear masks and maintain social distancing when possible.  The Core can provide Delta masks if researchers need to be less than 6 feet apart for training or other activities.  

Overview of Services

The goal of the Experimental Pathology Laboratory (ExpPath) is to assist researchers with the molecular and morphologic characterization of normal and disease tissues in support of biomarker discovery and translational research. The primary focus of the laboratory is on animal models of disease, but ExpPath also works closely with the Center of Biospecimen Research and Development (CBRD) supporting downstream analyses for non-CLIA/CAP human research studies. ExpPath offers a wide range of services as well as guidance and support for the design and execution of histopathological experiments. Researchers are provided training that follows "best practices" of current methods and techniques, and are provided access to microscopes and state-of-the art instrumentation.

The three components of the Laboratory are:

1. Histologic processing of animal tissues (Paraffin; frozen; vibratome and whole organ clearing)
2. Biomarker staining (immunohistochemistry, Opa-based IF multiplexng & RNA in situ hybridization)
3. Tissue imaging and analysis (basic microscopy; whole slide scanning; multispectral imaging; and laser capture micro-dissection).

Histologic processing and standard immunohistochemistry of human tissues are done through the CBRD. Experimental Pathology continues to support RNA in situ hybridization (chromogenic and fluorescence) assays, multiplex immunofluorescence staining, and laser capture microscopy for both animal and non-CLIA/CAP human research studies. ExpPath also provides high throughput whole slide scanning, multispectral imaging and image analysis of both human and animal tissue sections.  

Investigators should discuss all projects involving both the CBRD and ExpPath (e.g. RNA ISH of human tissue sections) with staff in both groups (Dr. Cindy Loomis for ExpPath and Ms. Iris Sydney or Drs. Andre Moreira and Luis Chiriboga for the CBRD) prior to inititating the project. Separate CBRD and ExpPath iLab forms should be filled out for the relevant services. 

New Core Services:

1. 10X Visium Spatial Transcriptomics in collaboration with GTC and the CBRD
2. Nanostring Digital Spatial Profiling (GeoMx) in collaboration with GTC and the CBRD
3. CODEX high-parameter multiplex IF (coming soon)

 For general questions:

        Regarding the Experimental Pathology Research Laboratory contact: Cindy Loomis (Director) at (646) 732-5828

For specific questions:

1. Histopathology offerings contact: Mark Alu at 212-263-3888
2. Biomarker staining and imaging services contact: Branka Dabovic or Priya Selvaraj at 212-263-3888.
3. Image analysis and microscopy services (including multispectral imaging): contact Valeria Mezzano at Valeria.Mezzanorobinson@nyulangone.org.

Alternatively, you can email us at #ExpPath@nyulangone.org  or use specific individual emails in the table below.

 The Experimental Pathology Laboratory collaborates with investigators to produce high quality tissue samples for animal model and clinical research; services include:

• Tissue preparation for animal models
• Processing & embedding for animal tissues
• Paraffin, cryo, and vibratome sectioning for animal tissues
• Hematoxylin and eosin staining for animal sections
• Immunohistochemistry of animal sections
• Special histochemical stains for both animal and human tissues
• Multiplex immunofluorescence for both animal and human tissues
• RNA in situ hybridization for both animal and human tissues
• Whole Slide Scanning and multispectral imaging for both animal and human tissues
• Image analysis using InForm, Visiopharm, FCExpress, Image J, and R platforms.
• Instrument Rental

By placing orders in iLab, user agrees to and accepts the Terms and Conditions governing the provision of shared core biomedical research services (“Services”) by NYU Grossman School of Medicine (“NYU”).

The Services are provided by NYU on an "AS IS" and "AS AVAILABLE" basis and without any express or implied warranties.

Leadership

Cynthia Loomis, MD, PhD
Director
Phone: 646-732-5828
Email: cindy.loomis@nyulangone.org 

Location and Hours of Operation

Links and Resources

1. NYU School of Medicine’s Experimental Pathology Research Laboratory
2. Yahoo Flickr Photos From Experimental Pathology

Contacts

Map